Composite
Part:BBa_K4895996:Design
Designed by: Patrick Jiang, Rori Hoover Group: iGEM23_ASU (2023-10-11)
Micro-Knock-Out-Plasmid Insert 1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part includes a 45bp region of homology to Insert 2, as well as a 50bp region of homology at the end to insert 2. The two regions of homology are set up for easy assembly, and are set in the recombination zone and tetracycline resistance to avoid messy recombination in the terminator or promoter sites.
Source
This part contains portions of tetracycline resistance, as well as two constitutive terminators: A T7 terminator and BBa_B1006, a constitutive promoter from iGEM.