Composite

Part:BBa_K4895996:Design

Designed by: Patrick Jiang, Rori Hoover   Group: iGEM23_ASU   (2023-10-11)


Micro-Knock-Out-Plasmid Insert 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part includes a 45bp region of homology to Insert 2, as well as a 50bp region of homology at the end to insert 2. The two regions of homology are set up for easy assembly, and are set in the recombination zone and tetracycline resistance to avoid messy recombination in the terminator or promoter sites.


Source

This part contains portions of tetracycline resistance, as well as two constitutive terminators: A T7 terminator and BBa_B1006, a constitutive promoter from iGEM.

References